Journal: The Journal of investigative dermatology
Article Title: Mediators of Capillary-to-Venule Conversion in the Chronic Inflammatory Skin Disease Psoriasis.
doi: 10.1016/j.jid.2022.05.1089
Figure Lengend Snippet: Figure 3. MCAM and its binding partners are specifically upregulated in blood capillaries in psoriasis. (a) Gene expression patterns of MCAM, LAMA4 and KDR in each BEC subtype in healthy and psoriatic conditions. Clusters with significant differences in expression (logFC > 0.2, adjusted P < 0.05) are marked by asterisks. Representative immunofluorescence images showing elevated protein levels of (b) MCAM (green), (e) LAMA4 (green) and (h) KDR (green) in psoriatic (lower) versus in healthy (upper) dermal capillaries stained for CD31 (white) and RGCC (red) as denoted by arrowheads. Bars ¼ 100 mm. Magnified images of the regions of interest (dotted outline) can be found in Supplementary Figure S3. The MFI of (c) MCAM, (f) LAMA4 and (i) KDR in CD31þRGCCþ blood
Article Snippet: Subsequently, the sections were incubated at 4 C overnight with the respective primary antibodies, including mouse antihuman CD31 (1:50, M0823, Dako, Santa Clara, CA), rabbit antihuman RGCC (1:100, HPA063701, Atlas Antibodies, Bromma, Sweden), goat antihuman MCAM (1:50, AF932, R&D System, Minneapolis, MN), sheep antihuman LAMA4 (1:100, AF7340, R&D System), goat antihuman KDR (1:20, AF357, R&D System), rabbit antihuman ERG (1:50, ab92513, Abcam, Cambridge, United Kingdom), mouse antihuman IL-17A (1:50, 14-7179-82, Invitrogen, Waltham, MA), goat antihuman ACKR1 Journal of Investigative Dermatology (2022), Volume 142 (1:100, NB100-2421, Novus Biologicals, Littleton, CO), mouse antihuman VWF (1:50, M0616, Dako), biotin-conjugated goat antihuman LYVE-1 (1:100, BAF2089, R&D System) and mouse antihuman podoplanin (1:100, M3619, Dako).
Techniques: Binding Assay, Gene Expression, Expressing, Staining
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